Effects of Nodal Position and Growth Regulators on In Vitro Growth of Dog Rose (Rosa canina)

Authors

  • A. Motallebi-Azar Department of Horticultural Sciences, Faculty of Agriculture, Tabriz University, Tabriz, Iran
  • F. Zaare-Nahandi Department of Horticultural Sciences, Faculty of Agriculture, Tabriz University, Tabriz, Iran
  • M. Matloobi Department of Horticultural Sciences, Faculty of Agriculture, Tabriz University, Tabriz, Iran
  • M. Shirdel Department of Horticultural Sciences, Faculty of Agriculture, Tabriz University, Tabriz, Iran
Abstract:

Dog rose (Rosa canina) has been using as a rootstock for ornamental roses also it is one of the medicinal plants. It can be propagated under in vitro conditions. After removing chilling requirement of buds, axillary wood buds of dog rose were cut in three nodal positions (lower, middle and terminal) on the stem and then explants after decontamination, cultured on Murashige and Skoog medium (MS medium) supplemented with 1 mg l-1 GA3 and 1 mg l-1 BAP. For shoot proliferation, MS medium supplemented with 1 mg l-1 GA3 and different concentration of PGRs. The results showed that maximum bud break percentage and highest shoot length were observed in lower nodal position. Minimum bud break percentage and shoot length were observed in middle nodal position. In the media with different concentrations of PGRs the highest shoot length was observed in combinations of BAP 1 mg l-1 and Ads 2 mg l-1. The large number of node and maximum axillary shoot percentage were observed in BAP 6 mg-1. The growth of dog rose was affected by different explants nodal position and growth regulators on in vitro. Assessment of BAP and Ads on axillary shoot percentage and node number was more effective than kinetin + TDZ and BAP + TDZ combination. In media with low concentration of BAP, TDZ and NAA and so kinetin, TDZ and NAA did not produce any axillary shoots or elongated shoots.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

effects of nodal position and growth regulators on in vitro growth of dog rose (rosa canina)

dog rose (rosa canina) has been using as a rootstock for ornamental roses also it is one of the medicinal plants. it can be propagated under in vitro conditions. after removing chilling requirement of buds, axillary wood buds of dog rose were cut in three nodal positions (lower, middle and terminal) on the stem and then explants after decontamination, cultured on murashige and skoog medium (ms ...

full text

The effect of medium and plant growth regulators on micropropagation of Dog rose (Rosa canina L.)

Dog rose (Rosa canina L.) is one of the most important ornamental and medicinal plants which are used as a rootstock for ornamental roses such as Rosa hybrid and Rosa floribunda. In vitro propagation of rose has a very important role in rapid multiplication of species with desirable traits and in production of healthy and d...

full text

the effect of medium and plant growth regulators on micropropagation of dog rose (rosa canina l.)

dog rose (rosa canina l.) is one of the most important ornamental and medicinal plants which are used as a rootstock for ornamental roses such as rosa hybrid and rosa floribunda. in vitro propagation of rose has a very important role in rapid multiplication of species with desirable traits and in production of healthy and disease-free plants. micropropagation of rosa canina l. was revised, usin...

full text

Effect of Antioxidants, Amino Acids and Plant Growth Regulators on in vitro Propagation of Rosa centifolia

Background: Rosa centifoliais commercially propagated by asexual means but in vitro propagation ensure the production of disease free and healthy plants and browning of explants creates hurdle in their multiplication. Objectives: The aim was to reduce oxidative browning of shoots of R. centifolia in MS medium during in vitro propagation. Materials and Methods: Axillary buds of R. centifolia we...

full text

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

Genetic diversity of dog rose (Rosa canina L.) using ISSR markers

Dog Rose is one of the most important species in Rosaceae family used as a medicinal plant and a rootstock for ornamental roses. This species is native to Iran, therefore, identification of indigenous genotypes of this species is important for genetic preservation or breeding purposes. Genetic diversity estimation of plant materials is one of the important pre-breeding activities in breeding cr...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 3  issue 1

pages  9- 17

publication date 2015-12-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023